The complete amino acid sequence of the cycle 3 mutant lab is shown in Fig. GFP was sheet isolated sheet from the jelly fish Aequorea victoria. The mutant form used in the pGLO plasmid is called the cycle 3 mutant , has three point mutations: phenylalanine100, threonine, , valine164, which were mutated to serine, methionine154 alanine. Learn vocabulary summary terms, , more with lab flashcards, games, other study tools. Demonstrate the power of genetic transformation! ” A gene is a piece of DNA that provides summary the instructions for making ( it sheet codes for . Ideal for AP Biology Lab 6. Pglo lab summary sheet. Start pglo studying Spring Summary Sheet. Students will glow with excitement when they transform bacteria with pglo pGLO plasmid. 4 ( pglo Crameri et al. Lab 9 - Transformation Worksheet Author: Biological lab Sciences pglo Last modified by:.
The plasmid contains several reporter genes lab most notably for the green fluorescent protein ( GFP) the ampicillin resistance gene. In this lab you summary will perform a genetic transformation which literally means “ change caused by summary genes. Biology of the pglo Cell Lab ( summary BIOLTransformation Lab Worksheet. Spray down workspace with bleach solution. The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms.
pGLO Transformation & Purification LAB Name_ _ _ _ _ Students use a simple procedure to transform bacteria with a gene that codes for Green Fluorescent Protein ( GFP). Michelle Landreville Biotechnology Unit 1: The lab techniques and analysis of the data. Introduction to Biotechnology Unit: Students will demonstrate knowledge of biotechnology concepts and apply knowledge to real- world problems in inquiry- based laboratories. Emphasis will be on lab technique and analysis of the data. Remove the rack containing the tubes from the ice and place on the lab counter. With a L micropipette with a sterile tip, transfer 250 L of LB nutrient broth to the + pGLO tube.
pglo lab summary sheet
Close the tube and gently tap with your finger to mix. Repeat with a new micropipette tip for the – pGLO tube. Complete the remainder of the activity sheet you began on day 1 of the lab.